Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore. increasing gRNA sequence diversity negatively affects gRNA distribution. leading to libraries of heterogeneous quality. https://www.alarecre.com/Wizarding-World-Harry-Potter-Scar-and-Glasses-iPhone-13-Pro-MagSafe-Case-p90262/